This biotechnology laboratory analyzes the effect of genetic information transfer through bacterial gene alternation in E. coli (Spilios, 2014). This alteration occurs through plasmid DNA transcribing the new genetic components into RNA, which will result in an amino acid (Sadava et al., 2014). This newly transcribed amino acid is an enzyme that will give transformed E. coli cells resistance to the antibiotics, beta-lactamase (Greenfield et al., 2009). The plasmid DNA of interest will be modified to become more resistant to the antibiotic Ampicillin, as beta-lactamase may break down the Ampicillin. In addition to plasmid DNA, bacteria contain other important features such as the reporter gene. This reporter gene will serve as an aid in observing the effect of the alteration, since this particular gene can be distinguished when a plasmid with foreign DNA is transferred from one plasmid to another (Spilios, 2014). Furthermore, the reporter gene used in this laboratory, Green Fluorescent Protein, is used to determine genetic resistance to Ampicillin. GFP would be useful in this experiment, as it would light up when the arabinose operon is present. Ampicillin is a derivative of penicillin that inhibits bacterial growth by interfering with the synthesis of bacterial cell walls. Since the E. coli is gram negative and Ampicillin kills gram-negative bacteria by synthesizing them with the cell wall, E. coli should not die without any transformation. However, the Ampicillin resistance gene is the beta-lactamase enzyme, which is secreted from transformed cells into the surrounding environment where it destroys Ampicillin (Dörr, 2010). To resist Ampicillins, E.coli uses the pGLO plasmid to protect the cell from Ampicillin invasion. There are four components that...... middle of paper ......n are resistant to Ampicillin and are able to decompose Ampicillin, while the untransformed gene would die due to Ampicillin damaging the cell wall of the bacterium. Furthermore, the presence of the arabinose operon would favor the binding of RNA polymerase and the transcription of GFP genes, making the bacteria glow under UV light. The result of this experiment confirmed the hypothesis. As explained in the results section, the transformed bacteria were alive, while the non-transformed bacteria were dead. Furthermore, one that contained the arabinose operon grew under UV light, while one without the arabinose operon did not grow under UV light, since GFP was not transformed by the arabinose operon. The sample with -pGLO LB was expected to have lawn, since nothing inhibits the growth of the cell. On the other hand, the sample with -pGLO LB/amp had no colonies, as the