The rapid, environmentally friendly, non-pathogenic and economical protocol of using plants to produce silver nanoparticles has attracted the attention of scientists because it provides a single-step technique for the processes of the biosynthesis method. Say no to plagiarism. Get a tailor-made essay on "Why Violent Video Games Shouldn't Be Banned"? Get an original essay Combination of biomolecules such as proteins, enzymes, polysaccharides, alkaloids, amino acids, tannins, phenols, saponins, terpenoids and vitamins, which are already found in plant extracts that have all the medicinal values ​​but are complex in chemical structures. For the synthesis of nanoparticles the protocol includes: Collection of the plant part and careful washing two or three times with tap water; followed by using distilled water to remove any debris. The cleaned part of the plant will be dried in the shade for 10-15 days and after drying, using a home blender, it will be pulverized. To prepare the vegetable broth, 20 g of dried powder is boiled in distilled water following the hot percolation method. The result is then filtered until no more material appears in the broth. In the AgNO3 solution added to the plant extract, the reduction of ions would be monitored using UV-visible spectra (Ahmed et al., 2016) Antimicrobial property of silver nanoparticles and its mechanism Against a wide range of more than 650 microorganisms, the Silver is a well-known antimicrobial agent against several classes of bacteria, gram-negative and positive viruses or even fungi. Recently silver is used in the form of nanoparticles and among all the other metals with antimicrobial properties it has been discovered that silver has the most effective action against bacteria and is also the least toxic for the environment. With these characteristics and good antimicrobial effect, silver nanoparticles are synthesized using various plant extracts against different microbes (Ahmed et al., 2016). The antimicrobial properties of silver nanoparticles depend on the size and environmental conditions such as size, pH and ionic strength and also capping agent. Silver must be in its ionized form to have antimicrobial properties. Silver ions are formed with nucleic acids and interact with nucleosides. All forms of silver are one-way sources of silver ions; these ions are incorporated with substances. There are references showing that the electrostatic attraction between nanoparticles and bacterial cells suggests it is the most preferable bactericidal agent. It is shown how nanoparticles enter the membrane and penetrate the cell causing damage to its cell wall. The reason for its antibacterial property is its ability to cause bacterial cell lysis. (Ahmed et al., 2016) Please note: This is just a sample. Get a custom paper from our expert writers now. Get a Custom Assay Gram-positive bacteria are less likely to be a silver target than Gram-negative bacteria. This is due to the peptidoglycan molecules in the cell wall; it is thicker than the cell wall of gram-negative bacteria. Both silver nanoparticles and silver ions, by interfering with disulfide bonds, can modify the three-dimensional structure of proteins. Green synthesis compared to chemical and physical method is making progress because it is cost-effective, non-toxic, and there is no need to use high-energy and toxic materials. (Ahmed et al., 2016)